Laboratory

Protein Interactions and Networks

09–17 October 2017

Wellcome Genome Campus, Hinxton, UK

Summary

This popular course provides training in the latest laboratory techniques
and bioinformatics tools necessary to discover protein interactions and
understand protein function.

In addition to laboratory and computational training in individual
techniques, the course provides the opportunity to discuss how these
methods can be integrated/applied to complementary approaches and
strategies for the study of protein interactions and networks.

Along with expert instructors, the practical programme will include
lectures in topical areas as well as a panel of distinguished guest
speakers, who will present the latest research in the field.

Learning outcomes
After attending the course, participants should be able to:

  • Evaluate and apply the principles and practices of different analytical methods for studying protein interactions and networks
  • Identify the strengths, weaknesses and pitfalls of each method
  • Assess how complementary analytical techniques and approaches can be integrated to answer biological questions
  • Identify suitable methods for their own research questions

Feedback from previous courses

  • It was a great pleasure to be here. This represents one of the most significant experience as a PhD student. I hope to come back again. Muchas gracias por todo!
  • I am grateful for giving me such valuable opportunity to learn state-of-art techniques from experts in the field. I would like to contribute to the community by successfully applying the knowledge that I learned here.
  • I was very happy to attend this course. the course planning, organization and execution was excellant and was mentored nicely. the information and sceitific knowledge gained by me in this course will be of immediate use to me and fasten my research. I am also grateful for bursaries that enabled me to attend this course.
  • Thank you all for this wonderful experience!
  • Thank you very much for the very well organized, interesting and very instructive course, it was a pleasure to attend!
  • Thank you all for a truly interesting week, I’ve returned to the lab and already feel more confident and independent. I was probably the least experienced person on the course, and the intensive lab work has benefitted me massively!

Programme

The programme will include lecture and practical
laboratory/computer-based sessions covering the following topics:

Protein Complexes by Mass Spectrometry

  • affinity purification using TAP tag approach
  • protein identification by Mass Spectrometry

Yeast Two-Hybrid

  • rapid methods for making Bait and Prey clones
  • using mating assays to investigate specific interactions
  • library screening: faster ways to find new partners

Binary Protein Interactions/BIAcore

  • low affinity extracellular receptor-ligand interactions by AVEXIS
  • biophysical protein interaction parameters (binding constants and kinetics)

Protein Interactions by FRET microscopy

  • sample preparation
  • intensity-based FRET microscopy
  • Fluorescence Lifetime Imaging (FLIM)-based FRET microscopy
  • image/data analysis (e.g. ImageJ / Fiji, KNIME)

Bioinformatics

  • network and pathway visualisation (e.g. Cytoscape)
  • protein interaction prediction and analysis
  • data integration

Learning outcomes
After attending the course, participants should be able to:

  • Evaluate and apply the principles and practices of different analytical methods for studying protein interactions and networks
  • Identify the strengths, weaknesses and pitfalls of each method
  • Assess how complementary analytical techniques and approaches can be integrated to answer biological questions
  • Identify suitable methods for their own research questions

Instructors and speakers

Course instructors
Jyoti Choudhary Wellcome Trust Sanger
Institute, Hinxton, UK
Chris Sanderson University of Liverpool, UK
Gavin Wright Wellcome Trust Sanger Institute, Hinxton, UK
Anton Enright
European Bioinformatics Institute, Hinxton, UK
Fred S. Wouters
University of Göttingen, Germany

Guest speakers
Erich Wanker Max Delbrück Center for Molecular Medicine,
Germany
Nadia Martinez-Martin Genentech, USA
Edward L.
Huttlin

Harvard Medical School, USA
Anne-Claude Gavin EMBL, Germany

How to apply

Prerequisites
Applicants should be PhD students, postdoctoral scientists or
clinicians/healthcare professionals engaged in or soon to commence
relevant research.

Cost
The
course is subsidised by the Wellcome Genome Campus Advanced Courses
and Scientific Conferences Programme. This is a residential
course and
there is a fee of £1040 towards board and lodging for
non-commercial applicants. Please contact us for the commercial fee.

Additional limited bursaries are available (up to 50%
of the course fee)
and are awarded on merit. Please see the “Bursaries”
tab for details.

Applications
Applications for this course can be completed online. Places are limited
and will be awarded on merit. If you have any problems with the online
application process, please contact us.

Please note: Applications
must be supported by a
recommendation from a scientific or clinical sponsor (e.g. supervisor or
head of department). A request for a supporting
statement will be sent
to your nominated sponsor automatically during
the application process.
Applicants must ensure that their sponsor
provides this supporting
statement by the application deadline. Applications without a supporting
statement cannot be considered.

Deadlines
Deadline for Applications: Closed

Travel visas
Please contact the
event organiser if you require a letter to support a travel visa
application. Note that letters will be provided to confirmed attendees.

Non-European Economic Area or Swiss nationals may be required to have a
visa to enter the UK.
Early application is strongly advised, as this process can take 6-8 weeks
or longer.

Please visit the following websites for further information:
UK Border Agency website and information for general visitors and business
visitors.

Cost

Cost
The
course is subsidised by the Wellcome Genome Campus Advanced Courses
and
Scientific Conferences Programme. This is a residential
course and
there is a fee of £1040 towards board and lodging for
non-commercial applicants. Please contact us for the commercial fee.

Bursaries
Advanced Courses are subsidised for non-commercial applicants from
anywhere in the world. Additional, limited bursaries are
available (up
to 50% of the course fee) and are awarded on merit. If you would like to
apply for a
bursary, please complete the bursary section of the online
application
form.

Please note that both the applicant
and sponsor are required to provide
a justification for the
bursary as part of the application.

Bursary terms and conditions

UK Courses (held at the Wellcome Genome Campus, Hinxton,
Cambridge)
A
limited number of bursaries are available for each course. These are
awarded by the selection committee according to merit. The bursary
covers a maximum of 50% of the course fee, though in exceptional
circumstances an application for the total course fee may be considered.
Where there are many bursary applications, the selection committee may
issue smaller amounts. We cannot assist with travel costs to attend UK
courses.

Overseas Courses (held outside of the UK)
A
limited number of bursaries are available for each course. These are
awarded on merit to cover travel, accommodation and sustenance. The
maximum award for travel (economy class) will be £750.

Bursaries can be applied for as part of the course application form.
Applicants
will be notified of a bursary award along with their place on
the
course, usually within one month of the application deadline. The
decision of the selection committee is
final.

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